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Image Search Results
Journal: Cells
Article Title: Development of a 3-Dimensional Model to Study Right Heart Dysfunction in Pulmonary Arterial Hypertension: First Observations
doi: 10.3390/cells10123595
Figure Lengend Snippet: Schematic of the protocol for the differentiation of cardiomyocytes from human iPSCs and complete EHT experimentation. ( A ) Cardiac differentiation consisted of a first step with 20 ng/mL of BMP4, 20 ng/mL of activinA, and 1.5 µM of CHIR99021 for mesoderm induction, followed by a second step with 5 µM of XAV939 for WNT signal inhibition and cardiac specification, and finally, insulin was added from day 8, after the first beating areas were observed. ( B ) The experimental design started with iPSCs cardio-differentiation and baseline functional measurements before the iPSC-CMs were collected and used for EHT production; later, EHT contractility was measured twice a week for 28 days until the end of the experiment.
Article Snippet: In short, a confluent monolayer of human iPSCs was differentiated by adding RPMI media (Thermofisher Scientific) supplemented with B27 minus insulin (Thermofisher Scientific), 50 μg/mL of ascorbic acid (Thermofisher Scientific), 20 ng/mL of BMP4 (R&D Systems, Minneapolis, MN, USA), 20 ng/mL of
Techniques: Inhibition, Functional Assay